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Covalab Inc
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Cytoskeleton Inc
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Novus Biologicals
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Thermo Fisher
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OriGene
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Santa Cruz Biotechnology
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Image Search Results
Journal: PLoS ONE
Article Title: Functions of Nonmuscle Myosin II in Assembly of the Cellular Contractile System
doi: 10.1371/journal.pone.0040814
Figure Lengend Snippet: (A,B) Cell surface topography revealed by platinum replica EM of non-extracted untreated (A) or 100 µM blebbistatin (BS)-treated cells (B). (C,D) Fluorescence microscopy of phalloidin-stained F-actin and immunostained α-actinin in detergent-extracted untreated (C) or 100 µM blebbistatin-treated cells (D). (E) Fractions of the cell perimeter occupied by lamellipodia and ruffles in untreated cells, cells treated with 75 µM or 100 µM blebbistatin, and cells recovering from treatment with 100 µM blebbistatin for indicated periods of time in minutes. Error bars, SD. (F,G) Fluorescence microscopy of phalloidin-stained F-actin and immunostained vinculin in detergent-extracted untreated (F) or 100 µM blebbistatin-treated (G) cells. Boxed regions in C, D, E, and G are zoomed in bottom panels. Scale bars, 2 µm (A,B) and 20 µm (C,D,F,G).
Article Snippet: The following primary antibodies were used:
Techniques: Fluorescence, Microscopy, Staining
Journal: PLoS ONE
Article Title: Functions of Nonmuscle Myosin II in Assembly of the Cellular Contractile System
doi: 10.1371/journal.pone.0040814
Figure Lengend Snippet: (1) NMII activation : Inactive NMII molecules diffuse to lamellipodia, where they are activated by double phosphorylation of MRLC. (2) Focal complex formation: Active unpolymerized NMII molecules bind actin filaments in lamellipodia and undergo the retrograde flow with them. If two NMII heads bind different actin filaments, one of which is anchored to a nascent adhesion, the resulting strain stabilizes the nascent adhesion and promotes its transformation to a focal complex. Long filaments in filopodia can encounter more NMII molecules increasing a probability of focal complex formation under filopodial bundles. (3) Assembly of NMII filaments: NMII molecules pulling on actin filaments attached to focal complexes experience a greater load, which triggers tension-dependent NMII polymerization at these sites. (4) Stress fiber formation: Multivalent NMII filaments exert large forces sufficient to promote maturation of focal complexes to focal adhesions. They also cross-link and align disordered actin filaments into bundles producing stress fibers. However, additional events, such as recruitment of α-actinin, are needed for the formation of semi-sarcomeric pattern in stress fibers.
Article Snippet: The following primary antibodies were used:
Techniques: Activation Assay, Transformation Assay